GATE 2026 Life Sciences Q40

Question

For a simple enzyme that follows Michaelis-Menten kinetics, kinetic data was collected in the absence (dotted line, -I), or presence (solid line, +I) of an uncompetitive inhibitor (I). Which one of the following Eadie-Hofstee plots best describes the expected result?
($v_0$ = initial velocity, [S] = free substrate concentration)

Accepted Answer

Diagram (D) showing $v_0$ vs $v_0$/[S] where the dotted line (-I) has a higher y-intercept and x-intercept than the solid line (+I), and the solid line (+I) has a less steep negative slope.

Answer

Diagram (A) showing $v_0$ vs $v_0$/[S] where the dotted line (-I) has a higher y-intercept and x-intercept than the solid line (+I), and both lines converge at the origin.

Answer

Diagram (B) showing $v_0$ vs $v_0$/[S] where the dotted line (-I) has a higher y-intercept and x-intercept than the solid line (+I), and both lines are parallel.

Answer

Diagram (C) showing $v_0$ vs $v_0$/[S] where the dotted line (-I) has a higher y-intercept and x-intercept than the solid line (+I), and the solid line (+I) has a steeper negative slope.